Insect cells, a new technology in agricultural biotechnology, are eukaryotic cells derived from insects. The in-vitro culture of insect cells is increasingly valued by scientists for two reasons: first, viruses with high host specificity, that can cause epidemics in insects but no harm to humans, livestock or plants, so it can be used as viral pesticides; second, the insect polyhedrosis virus, with high expression protein and gene fragments that can be used to construct recombinant viruses for the efficient expression of foreign genes, can be used for medicinal protein production.
Therefore, the in-vitro culture of insect cells is becoming an important means for the production of biological products for agriculture and human health care.
Insect cell lines are widely used in cell fermentation and fundamental research. Sf9 and S2, are generally semi-adherent cell lines which can bedetached from the culture plate by blowing and beating. But such may lead to cell aggregations and bring greater difficulty to the identification of cells in aggregations during the manual counting process. A good solution to this problem, the cell aggregation counting function of CountStar® can count cells in aggregates accurately and give a more accurate and reliable result.
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