Cell growth curve is a common method for measuring the absolute growth of cell number, an important indicator to determine cell viability and one of the basic parameters for the culture of the basic biological properties of cells. When the saturated density is reached, cell growth stops, a stable period begins, and then a decline stage. In order to accurately describe the dynamic change in the number of cells throughout the whole process, the typical growth curve can be divided into 4 parts: incubation period with slow growth; exponential growth phase with large slope, plateau phase and decline period. The cell growth curve can be obtained by plotting the number of living cells (10’000/mL) against the culture time (h or d).
The MTT (also known as 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) method is commonly used to plot the curve. Succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT into formazan, an insoluble blue purple crystal, which will cumulate in live cells but never in dead cells. The cell growth curve plotted with the MTT method only presents the relative number and viability of measured cells rather than the absolute number of measured cells, and the enzymatic reaction is affected by many factors. Therefore, the cell growth curve plotted on the basis of direct cell technology can reflect the growth of cells in a more accurate manner.
Countstar® automated cell counters have a powerful data management function to store all the experimental data. The operation is simple, as is the drawing of the cell growth curve. Realizing the analysis of the cell growth curve:
Overlay Analysis of The Cell Growth Curve
In cell culture, the study of pharmacology and toxicology often requires the overlay analysis of multiple growth curves in order to find the optimal culture conditions or the best dosage. Countstar® can directly call up multiple growth curves for comparative analysis.
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